A simple, fast, precise and accurate reverse phase HPLC method was developed for the simultaneous estimation of Nebivolol hydrochloride and Amlodipine besilate in tablet dosage forms. The separation was achieved by C18 Inertsil column {250 × 4.6 mm (id) 10 µm} in isocratic mode, with mobile phase containing methanol: Acetonitrile: 50 mM KH2PO4 buffer (25:30:45 v/v, pH 3.0) at a flow rate of 1.0 ml/min. UV detection was carried out at 267 nm. The retention time of Nebivolol hydrochloride and Amlodipine besilate was 7.63 min and 3.34 min respectively. Linearity of Nebivolol hydrochloride and Amlodipine besilate were found in the range of 10-100 µg/ml and 10-100 µg/ml respectively. The method can be used for routine quality control analysis of combination for these drugs in tablets.
Loading....